The germinal epithelium known as the germ layer is a group of cells formed during animal embryogenesis. Few primitive animal tissues derive from two germ layers, the endoderm and the ectoderm; while most animals derive from three germ layers, the ectoderm, mesoderm, and endoderm. The germ layers are known as the primary layers of cells in an animal embryo. As the embryo develops, the cells differentiate to form the different body tissues. In 1817, Caspar Friedrich Wolff was the first to observe the early organization of the embryo into leaf layers, while while studying chicken embryos, three primordial germ layers were discovered by Christian Pander. In 1871-1873, Robert Remak had further defined the concept of germ cell layer and introduced into English the terms mesoderm by Huxley in 1871 and ectoderm and endoderm by Lankester in 1873. The first major event of the embryonic period occurs after implantation, the three Primary germ layers known as ectoderm, mesoderm, and endoderm differentiate via the blastocyst into the inner cell mass. All tissues and organs in the body will develop from embryonic tissues. Germ layers form rapidly in humans and it is difficult to determine the exact sequence of events. As the embryo develops, the cells differentiate to form the different body tissues. After a sperm fertilizes an egg for about 8 days, several divisions occur in the lining of the uterus and begin to form three germ layers: ectoderm, mesoderm, and endoderm. The neural crest is known as the fourth germ layer because it is essential to the germ layers as they derive from the ectoderm. They are known as the embryonic tissues from which all tissues and organs are developed and which together will later constitute the body. The three compound germ layers are formed during the gastrula stage of development. The layers are determined by their physical position in the gastrula. This stage follows the zygote and blastocyst stages; The gastrula forms when the embryo is approximately 14 to 16 days old in humans. Epithelial tissues develop in the embryo from all three layers. Connective tissues and muscle tissues all come from the mesoderm. The ectoderm gives rise to nervous tissue. The endodermis is the innermost layer of the three germ layers of the developing embryo. These germ layers produce tissue in the pancreas, lungs, and thyroid. They form: the liver, the intestines, the stomach, the colon, the pharynx and the epithelial parts of the trachea..