Background of the studySystematics and taxonomy involve the identification and resolution of relationships between species. But as species are more taxonomically complex today, the integration of molecular technology as an alternative tool in species identification has allowed systematic studies to gain new perspectives in evolutionary studies. Taxonomy has always been at the forefront in the study of life and will be forever (Wheeler 2004). And with the increase in development in the field of molecular biology and genetics, DNA is now used as a means of species identification. DNA barcodes are labels along a specific genetic sequence that have been proposed for species identification. The use of DNA as a barcode developed by Hébert et al. (2003) was the system used due to its precise and stable method, whereby even a tiny sample of tissue is sufficient for the identification of this species (Laiou et al., 2013). DNA barcoding is used today as a universal system for identification and taxonomic clarification (Hollingsworth et al., 2011). The importance of DNA barcoding is evident as the pace of species discovery will emphasize sorting species from different taxon classifications representing new species (Hebert et al., 2005). Barcodes support biological applications such as the identification of medicinal plants and even endangered plants (Muellner et al. 2011). DNA barcoding defines the expansion and discovery of the world's ever-expanding inventory of species diversity. DNA barcoding requires it to be standard, scalable and minimal. The low rate of nucleotide substitution in the plant mitochondrial gene has led to a major debate regarding a plant's identity code. CO1 was the standard gene used for ...... middle of article ...... plastid atpB and rbcL gene sequences. Systematic Biology 49: 306-362. doi:10.1093/sysbio/49.2.306 Tezcan, M., Vlachonasios, K. and Aki, C. (2010) DNA barcoding study of the Sideritis Bornm Trojan. A medicinal plant endemic to Ida Mountain, Turkey. Fresenius Environmental Bulletin, PSP vol.19-no.7Tamura, K., Peterson, D., Peterson, N., Stetcher, G., Nei, M. and Kumar S. (2011) MEGA5: Molecular Evolutionary Genetic Analysis Using Methods maximum likelihood, evolutionary distance and maximum parsimony. Molecular biology and evolution. Retrieved February 1, 2014 from http://mbe.oxfordjournals.org/Wang, Q., Yu., QS., Liu, JQ. (2011) Are nuclear loci ideal for barcode factories? A case study of genetic delimitation of two sister species using multiple loci and multiple intraspecific individuals. Journal of Systematics and Evolution 49: 182-188Wheeler (2004)