IntroductionBacteria and archaea are around us and in different environments, even though we cannot see them without a microscope. One of the environments in which microorganisms live is the salty environment. The different types of microorganisms that live in the salty environment are halotolerant, halophilic and non-halophilic. Halophilic microorganisms live and grow in environments with high salt content (Arai et al., 2014). There is also a non-halophile that can tolerate salt, but in small amounts it would not survive in high salt environments, whereas halotolerants can survive in a high salt environment, can tolerate some salt but they grow better without salt. saline environment (Brock et al., 2016). The microbes tested in this experiment apply to the previously mentioned salty, halotolerant, halophilic, and nonhalophilic microorganisms. If different cultures were provided with three different microorganisms, it is possible to determine the culture based on salinity and temperature. If 15% salt is used the only expected results should be halobacterium salinarium, if 10% salt is used the only expected results should be staphylococcus aureus and if 1% salt is used only the expected result should be serratia marcescense and staphylococcus aureus. .Materials and methodsFirst, a mixed broth containing H. salimarium, S, aureus and S. marcescens was provided by the laboratory instructor. Three plates were also provided, one containing 15% NaCl milk agar, one containing 10% NaCl nutrient agar, and the last one containing 1% NaCl nutrient agar prepared before the laboratory. Using the mixing broth, a quadrant streak plate was made for each...... middle of paper ...... optimally when the salt concentration is 15% and the temperature is 37°C (Grant, Kamekura, McGenity, & Ventosa, 2015). Serratia Marcescens grows optimally when the salt concentration is 1% and the temperature is 37°C. Staphylococcus Aureus grows optimally at a salt concentration of 1% and can tolerate up to 10% while the optimal temperature is 37°C. Isolations of microorganisms were successfully obtained in the plates. In 1% NaCl, two organisms were isolated and identified: Serratia Mercescens and S. Aureus. In 10% NaCl, the only organism isolated was Staphylococcus Aureus. In the last plate, Halobactirium Salinarium 15% milk agar was isolated. Using these plates containing salt and milk agar it was possible to isolate two different types of microorganisms and in one of the plates it was possible to isolate two types of microorganisms..